ملزومات نمونه توالی یابی کامل ژنوم


🔸مقدمه 🔸روش کار 🔸ملزومات نمونه 🔸سوالات متداول


Whole Genome Sequencing

Sample Type: Genomic DNA with no degradation or RNA contamination

Sample Purity (OD260/280): 1.8-2.0

Recommended Quantity

for plant and animal : 500 µg-1 mg

For prokaryotes: ≥5 µg, ≥100 ng/ µl

Sample concentration:

200bp/350 bp/500bp library ≥30 ng

2 kb/ 5kb/ 10kb/ 20 kb library ≥133 ng

Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)